NMR Instructions

This website has an excellent explanation of how NMR works: https://www2.chemistry.msu.edu/faculty/reusch/OrgTxtBook/Spectrpy/nmr/nmr1.htm 

Sample Preparation

General sample concentration: 25 mg (or 25 uL) sample in ~ 1.0 mL of deuterated chloroform.

We want to keep the deutrated solvent clean. These instructions are written to minimize the chances of contamination.

1. Transfer 25 mg of solid or use a micro pipette to transfer 35 uL of your sample into a small plastic vial

2. Set the plastic vial into one of the orange holders.

3. Get a new glass pipet and bulb. You'll use this to transfer the deuterated chloroform. 

4. Bring the deuterated chloroform bottle close to your plastic tube and loosen the lid. 

5. Use the glass pipet and rubber bulb to transfer about 1.0 mL of deuterated chloroform to the plastic vial where you already added your sample.

6. Read the volume on the side of the vial. 

   1. If you need more solvent, get a new glass pipet.

   2. Otherwise, close the chloroform bottle and use the pipet to mix and then transfer your sample to an NMR tube

7. Cap the NMR tube and slide on an autosampler collar. The autosampler collar should be adjusted to the top of the NMR tube so that it stops at the cap. You can check the depth in the metal autosampler depth gauge.

8. Wipe down the outside of the NMR tube with a kimwipe.

9. Record your sample in the instrument notebook and place your sample in the NMR carosel.